This study was to find out the isolated and combined effect of ladder and medicine ball training on agility among handball players. To achieve the purpose of the study Visvesvaraya Technological University inter collegiate 120 male handball players except goalkeepers were randomly selected from different colleges in and around Karnataka state, India. During the academic year 2021-22. The age of the subjects will be ranged between 17 to 25 years. The subjects will be selected in handball and only those who represented their respective college team are taken as subjects and divided into four equal groups of 30 subjects each. Group - I will perform Ladder training, Group - II will undergo Medicine ball training, Group - III will undergo Ladder & Medicine ball training and Group - IV act as controlled. All the subjects are informed about the nature of the study and their consent is obtained in order to get their co-operation on till the end of the experimental and training period. The subjects were tested on agility before and after the training period. Prior after the training period agility were measured by using shuttle run test. Analysis of Covariance (ANCOVA) was applied as statistical tool for the present study. The Scheffé S test was used as post-hoc test at whatever point the ‘F’ - ratio of the adjusted post-test means were discovered to be significant at 0.05 level of significance. Both, ladder, medicine ball training and combined training group influence on agility when compared with control group. Combined (ladder and medicine ball training) may have better influence on agility of hand ball players.